Education
PhD in Biology (Aquatic Ecology/Environmental Monitoring) - Ongoing
Queen's University, Kingston, Ontario
Project Title: Monitoring the biodiversity of Southern Ontario with eDNA and UAV
Principal Investigators: Dr. Yuxiang Wang and Dr. Stephen Lougheed

MSc in Biology (Limnology/Phycology/Environmental Monitoring) - Mar 2021
Queen's University, Kingston, Ontario
Project Title: Characterizing the development of cyanobacterial blooms with UAV and eDNA
Principal Investigators: Dr. Yuxiang Wang and Dr. Daniel Lefebvre
BScH with Distinction (Biology/Psychology Specialization) - June 2018

Publications
Tournayre, O., Tian, H., Lougheed, D., Windle, M. J. S., Lambert, S., Carter, J., Sun, Z., Ridal, J., Wang, Y. X., Cumming, B. F., Arnott, S. E., Lougheed, S. C. 2024.  Enhancing metabarcoding of freshwater biotic communities: A new online tool for primer selection and exploring data from 14 primer pairs. Environmental DNA, 6(4), e590. DOI: https://doi.org/10.1002/edn3.590
Tournayre, O., Tian, H., Maracle, S., Lougheed, S. C. 2024. An overview and simple guide to environmental DNA protocols and workflows. Version 2.2.1. Queen’s University, Kingston, Ontario, Canada. DOI: https://doi.org/10.5281/zenodo.13421371
Chen, Y., Tournayre, O., Tian, H., Lougheed, S. C. 2023. Breeding phenology of a threatened frog species using eDNA and automatic acoustic monitoring. PeerJ, 23(11), e14679. DOI: https://doi.org/10.7717/peerj.14679
Tian, H. (eds). 52nd Ontario Ecology, Ethology, & Evolution Colloquium Proceedings. 2022.
Conference proceedings. Link: https://allensgalleryca.wordpress.com/2023/02/01/oe3c-2022/
Tian, H., Jin, J., Chen, B., Lefebvre, D. D., Lougheed, S. C., Wang, Y. X. Depth-dependent
spatiotemporal dynamics of overwintering pelagic Microcystis in a temperate water
body. Microorganisms 2021, 9, 1718. https://doi.org/10.3390/microorganisms9081718

Manuscripts in Progress
Tian, H., Griffin, D., Lougheed, S. C., Wang, Y. X. A novel loop-mediated isothermal amplification (LAMP) assay for highly specific field detection and identification of the Boreal Chorus Frog (Pseudacris maculata). Manuscript in progress.
Tian, H., Liu, J. L., Lougheed, S. C., Wang, Y. X. Development and validation of loop mediated isothermal amplification (LAMP) primers for detecting invasive species in Eastern Ontario. Manuscript in progress.
Tian, H., Chen, Y., Wang, Y. X., Lougheed, S. C. A novel, low-cost method for measuring
chlorophyll-a from eDNA extraction waste. Manuscript in progress.

Conference Presentations

River Symposium - Oct 2022
Hosted by the St. Lawrence River Institute, in Cornwall, Ontario
Oral Presentation: Loop mediated isothermal amplification (LAMP) primers for the rapid
detection of 12 invasive species in Eastern Ontario

Canadian Herpetology Society Annual Conference - Sept 2022
Hosted by the Canadian Herpetological Society, in Fredericton, New Brunswick
Oral Presentation: Using droplet digital PCR to assess breeding phenology in chorus frogs in Ontario
Biology Graduate Research Symposium - Sept 2022
Hosted by the Department of Biology, Queen's University in Kingston, Ontario
Poster Presentation: Surveying all aquatic biodiversity at once using an environmental
DNA approach

Lake and Trails Festival - July 2022
Hosted by and in the Township of Sydenham, Ontario
Poster Presentation: Surveying aquatic biodiversity in South Frontenac County using
environmental DNA

Queen's University Biological Station Open House - June 2022
Hosted by the Queen's University Biological Station, in Perth, Ontario
Poster Presentations (2): Surveying all aquatic biodiversity at once using an
environmental DNA approach; Revealing chorus frog breeding phenology using eDNA
and acoustic monitoring techniques

Ontario Ecology, Ethology, and Evolution Colloquium - May 2022
Hosted by me, in Kingston, Ontario
Poster Presentation: Developing novel citizen science protocols for environmental DNA based biodiversity surveys

Queen's University Biological Station Open House - June 2021
Hosted by the Queen's University Biological Station, in Perth, Ontario
Poster Presentations (2): Surveying all aquatic biodiversity at once using an
environmental DNA approach; Revealing chorus frog breeding phenology using eDNA
and acoustic monitoring techniques

Fulford Water Symposium - Feb 2021
Hosted by the Queen's University Biological Station and University of West Indies, over Zoom
Oral Presentation: Early detection of toxic algal blooms using unmanned aerial vehicles

Limnology Seminar Series - Sept 2020
Hosted by the Department of Biology, Queen's University, over Zoom
Oral Presentation: Characterizing the biotic and abiotic predictors of cyanoHABs with environmental DNA and UAV imaging

Lake Links 18th Annual Workshop - Oct 2019
Hosted by Watersheds Canada in Perth, Ontario
Poster Presentation: Monitoring Blue-Green Algae in Dog and Cranberry Lake with UAV and Water Sampling

Lower Cataraqui Water Quality Workshop - June 2019
Hosted by the Young Lab, University of Ottawa in Perth, Ontario
Panelist/Roundtable discussion participant

River Institute 26th Annual Symposium - May 2019
Hosted by the St. Lawrence River Institute in Cornwall, Ontario
Roundtable discussion participant

Interdisciplinary Freshwater Harmful Algal Bloom Workshop - Apr 2019
Hosted in Toronto, Canada
Poster Presentation: Characterizing the early development of cyanobacterial algal blooms in small freshwater bodies through high temporal frequency eDNA and photogrammetric analysis with UAV

Biology Graduate Research Symposium - Mar 2019
Hosted by the Department of Biology, Queen's University in Kingston, Ontario
Oral Presentation: Characterizing cyanobacterial bloom development through consumer-grade UAV imaging and water sampling
Poster Presentation: Monitoring cyanoHABs with low cost UAV and RGB imaging

Grants and awards
Sustainable Action Fund ($5000) - May 2024 to Ongoing
Queen's University AMS
Grant for constructing a barn swallow habitat at QUBS

Queen Elizabeth II Graduate Scholarship in Science and Technology ($15,000) - May 2024
Province of Ontario
Merit based scholarship for postgraduate studies
Best Teaching Demonstrator Award - March 2024
Biology Department, Queen's University
Award for best TA for 2023/2024

Doctoral Field Travel Award ($3000) - March 2024
Queen's University
Award for funding fieldwork

Ontario Graduate Scholarship ($15,000x2) - May 2022 to April 2024
Province of Ontario
Merit based scholarship for postgraduate studies

Lake Ecosystem Grant ($10,500) - Jan 2022 to 2024
County of South Frontenac
Research grant in collaboration with the Queen's University Biological Station, for
monitoring biodiversity in lakes in South Frontenac County, Ontario

Graduate Inclusivity Fellowship ($1000/$2000) - Sept 2020 to Sept 2022
Queen's University
Fellowship for improving inclusivity and graduate student representation at Queen's University

CSEE Regional Conference Grant ($500) - Mar 2022
Canadian Society for Ecology and Evolution
For the Ontario Ecology, Ethology, and Evolution Colloquium 2022

Scientific Meeting and Sustainable Conferencing Grant ($4,500) - Mar 2022
The Company of Biologists
For the Ontario Ecology, Ethology, and Evolution Colloquium 2022

Ages Foundation Scholarship ($5000) - Aug 2021
Rare Charitable Nature Reserve
For citizen science environmental DNA research

Principal's Student Initiatives Fund ($1000/$1000) - Dec 2020/Mar 2022
Queen's University
For outreach within the Queen's Biology Department and the Ontario Ecology, Ethology, and Evolution Colloquium 2022

Inclusive Community Fund ($2500) - Dec 2020
Queen's University
For improving inclusivity and diversity in the Queen's Biology Department

School of Graduate Studies Student Initiatives Fund ($500) - Dec 2020
Queen's University
For outreach within the Queen's Biology Department

NSERC-USRA ($4500) - May 2015
Queen's University
Undergraduate summer research award

Teaching
Queen's University Environmental DNA Workshop - July 2022, July 2023, May 2024
Queen's University
Course developer and logistics coordinator. I developed this workshop together with Dr. Stephen Lougheed, Dr. Yuxiang Wang, Dr. Orianne Tournayre, and fellow PhD student Stafford Maracle as part of Queen's University's new micro-credentials program. It is the first practicum based workshop of its kind on the emerging field of Environmental DNA. We collaborated with three notable scientists in the field, Dr. Robert Hanner (University of Guelph), Dr. Elizabeth Clare (York University), and Dr. Chris Wilson (MNR), and the companies Bio-Rad and New England Biolabs to deliver this workshop for three years. My role in this workshop included curriculum development, writing the manual, organizing logistics and communications, organizing practicums, and teaching. Over three years, we've taught over 50 participants, who all received certification in eDNA, one of the first in the world.

Experimental Approaches to Animal Physiology (BIOL 401) - Sept 2018 to Ongoing
Dr. Yuxiang Wang, Queen's University
Logistical Coordinator and Demonstrator. I have taught this course for over five years.. During this time, I have gone from assisting with grading to handling all course logistics, much of the procurement, lab module design, and syllabus. I now handle all student communications and feedback, manual development, supplies, and all grading. I have trained three department associates in running the setup and logistics for the labs.

Environmental Physiology of Animals (BIOL 322) - Jan 2021 to Ongoing
Dr. Yuxiang Wang, Dr. Barry Madison, Queen's University
Grading TA

Introductory Biology of Cells (BIOL 102) - Jan 2021 to April 2021
Dr. Kenton Ko, Queen's University
Tutorial TA

Mendelian and Molecular Genetics (BIOL 111) - May 2020 to Aug 2020
Dr. Laura Nagel, Queen's University
Tutorial TA

GIS & Spatial Analysis (ENVS 4010U) - Nov 2020
Dr. Robert Bailey, Ontario Tech University
Guest lecturer: UAV workflow and project design. I was invited to give this lecture on the applications of UAV in GIS workflows back in 2022.

Mendelian and Molecular Genetics (BIOL 205/206) - Sept 2018 to Ongoing
Dr. Ian Chin-Sang, Queen's University
Tutorial/Lab TA, Admin TA

QUBS Canada-China Exchange Field Course (BIOL 307/317) - July 2019 to Aug 2019
Dr. Yuxiang Wang and Dr. Stephen Lougheed, Queen's University
Logistics TA

General Chemistry (CHEM 112) - Jan 2019 to Apr 2019
Dr. Michael Mombourquette, Queen's University
Lead Lab TA

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